NNadir
NNadir's JournalWhile our nation focuses on yet another murderer,...
I admire Jacinda Ardern more and more.
She struck exactly the right tone, dressing in traditional Muslim attire, visiting with the victims, and refusing to acknowledge the murderer by refusing even to say his name.
Dopamine-based mechanism for transient forgetting
The paper I'm going to discuss in this post is...um...wait...I forget...oh yeah, this one: Sabandal, J.M., Berry, J.A. & Davis, R.L. Dopamine-based mechanism for transient forgetting. Nature 591, 426430 (2021)
An excerpt from the introductory text:
One form of active forgettingknown as intrinsic forgettinginvolves one dopamine neuron (DAN) that innervates the γ2α′1 compartment of the axons of mushroom body neurons (MBNs) and the dendrites of the downstream, compartment-specific mushroom-body output neurons (MBONs)4,5,6. This DAN resides in a cluster of 12 DANs in each brain hemisphere that is known as the protocerebral posterior lateral 1 (PPL1) cluster4. Current evidence indicates that the ongoing activity of these DANs after aversive olfactory conditioning slowly and chronically erodes labile and nonconsolidated behavioural memory5, as well as a corresponding cellular memory trace that forms in the MBONs6. This intrinsic forgetting mechanism is shaped by external sensory stimulation and sleep or rest7, and is mediated by a signalling cascade in the MBNs that is initiated by the activation of the dopamine receptor DAMB, which leads to the downstream activation of the actin-binding protein Cofilin and the postulated reorganization of the synaptic cytoskeleton1,8,9.
By contrast, there is little understanding of the mechanisms that arbitrate transient forgetting. Neuropsychological studies of failures or delays in retrieval in humans have primarily focused on lexical access. Phonological blockers or interfering stimuli produce a tip-of-the-tongue state10the failure to recall the appropriate word or phrase. Tip-of-the-tongue states are resolved when the distracting signals dissipate10. Several brain regions have been implicated in tip-of-the-tongue states from functional magnetic resonance imaging studies11, but the neurobiological mechanisms that produce a temporary state of impaired retrieval are unknown. Our study offers an entry point into this area of brain function...
I'm not saying that we should forget about this paper, but animal model discussed is flies:
I personally had no idea that flies could be trained with blue light. One learns something every day, and then one dies.
Some figures from the text:
Fig. 1: External stimuli transiently disrupt retrieval of PSD-LTM.
The caption:
Some more text ("DAN" is described above in the introduction, as dopamine neurons, as are PPL1 protocerebral posterior lateral 1 1:
Because DANs of the PPL1 cluster (PPL1 DANs) are involved in intrinsic forgetting, we asked whether they might also be involved in the processes that underlie transient forgetting. Pilot experiments demonstrated that strong, prolonged thermogenetic stimulation of all 12 PPL1 DANs per hemisphere (TH-D′>TrpA1 flies; for a full list of fly lines with their genotypes, see Supplementary Information) significantly reduced expression of PSD-LTM (Extended Data Fig. 2ac) even 24 h before retrieval. We observed the opposite effect upon blocking synaptic output from PPL1 DANs (TH-D′>Shibire), which suggested the existence of a memory reserve that remains hidden unless synaptic output from the DAN is suppressed (Extended Data Fig. 2c). We used these assays and a collection of split-gal4 fly lines to spatially restrict TrpA1 expression to subpopulations of the PPL1 DANs
As far as I'm aware, no protocerebral Republicans were injured in this study, only flies.
Some of the flies, had a mutant DAMB (dopamine receptor in mushroom bodies) - no mushroom fly on fecal mass related jokes are acceptable in the Science forum - and the mutants, as mutants do, behaved differently.
Fig. 2: Transient suppression of memory engages a single pair of PPL1 DANs and the dopamine receptor DAMB.
The caption:
In elegant work, the authors focused on a particular neuron in the fly and imaged. (Don't try this at home, but do, if you pray, pray for the graduate students who imaged fly neurons.)
Next figure:
Fig. 3: Stimulating PPL1-α2α′2 did not erase the 72-h PSD-LTM trace in MBON-α2sc.
The caption:
The DAMB protein expression was knocked down by using the abortifacient drug RU-486, which apparently results in the generation of interfering RNA, RNAi. (RNA is in the news these days, for good and for bad.)
There's a line in the wonderful movie Elizabeth where the character played magnificently by Geoffrey Rush remarks to the character played by Christopher Eccleston, the Duke of Norfolk, who at the moment that he is to become executed for his Catholicism, he remarks, "The people will remember me," whereupon the Rush character, Walsingham, says "No the people will forget."
Here, the flies will forget.
Fig. 4: Airflow, electric shock or blue light require PPL1-α2α′2 and DAMB function to cause transient forgetting.
The paper's conclusion:
However, the DAMB receptor is used for both permanent and transient forgetting. DAMB is widely expressed across the MBN axons12 but alters synaptic plasticity differently across MBN compartments29. It is possible that DAMB signalling may be distinct for the two forms of forgetting. DAMB preferentially couples with Gq, the knockdown of which inhibits the potent erasure of memory13, but its potential role in transient forgetting is unknown. The scaffolding protein Scribble orchestrates the activities of Rac1, Pak3 and Cofilin8, all of which are important for the permanent forgetting pathway (Extended Data Fig. 7). However, Scribble knockdown or inhibition of Rac1 does not enhance the PSD-LTM8,9 as is the case in DAMB-knockdown flies, which suggests that this scaffolding signalosome does not have a large role in transient forgetting. In summary, the two distinct forms of forgettingtransient and permanentshare a dopaminergic mechanism and a common dopamine receptor, but differ in upstream and downstream neural circuits and in downstream signalling pathways within MBNs.
OK, I'll say it. I'm drawn to papers focusing on obscure stuff like this like a fly on shit.
Have a nice evening.
On now: A conversation with Bill Clinton, on the future of Democracy.
https://ecornell.cornell.edu/keynotes/view/K031821b/Angel
The greenhouse gas emissions of indoor cannabis production in the United States
The paper I'll discuss in this post is this one: The greenhouse gas emissions of indoor cannabis production in the United States (Summers, H.M., Sproul, E. & Quinn, J.C. Nat Sustain (2021))
The abstract of the paper, which should be open sourced, says it all:
Some excerpts from the full text:
The initial amendment legalizing recreational cannabis in Colorado required the majority of cannabis product to be sold at a collocated retail location4. This restriction led to cultivation practices occurring within the city limits of Denver, CO. This, along with security, theft and quality concerns, consequently led to the cultivation of cannabis indoors. Although data concerning the exact amount of cannabis by cultivation method are not currently publicly available for the United States, a recent survey of producers in North America showed that 41% of respondents indicated that their grow operations occur solely indoors5. It is well known that indoor cannabis cultivation requires significant energy input, reflected in high utility bills and industry reports4,6,7,8,9. However, many of these large energy loads, along with other material inputs required to cultivate indoor cannabis, have not yet been equated to GHG emissions.
Previously, rudimentary quantifications of GHG emissions from indoor cannabis have been performed by equating emissions with electricity use from monthly bills6,7. However, this approach omits additional GHG emissions from other energy sources, such as natural gas, upstream GHG emissions from the production and use of material inputs, and downstream GHG emissions from the handling of waste. The most thorough report quantifying GHG emissions from indoor cannabis is from Mills10, which states that growing 1 kg of cannabis indoors releases 4,600 kg of carbon dioxide equivalent (CO2e). However, the scope of the work was intended to be a central estimate, representing a singular US location case study for the industrys general practices. Furthermore, Mills10 conducted this study prior to legalization and only used data from small-scale experimental systems, thus lacking validation of full-scale commercial grow operations...
...An indoor cannabis cultivation model was developed to track the necessary energy and materials required to grow cannabis year-round in an indoor, warehouse-like environment. This environment maintains climate conditions as required for the cannabis plants, yielding a consistent product regardless of weather conditions. The model calculates the necessary energy to maintain these indoor climate conditions by using a years worth of hourly weather data from more than 1,000 locations in the United States11. The analysed locations are independent of current legal status and represent hypothetical grow facilities in all 50 US states. The model then converts the required energy, supplied from electricity and natural gas, into GHG emissions through electrical grid emissions data from 26 regions in the United States12 and life cycle inventory (LCI) data13,14...
Here's a look some pictures from the paper:
Fig. 1: Life cycle GHG emissions and energy intensities from indoor cannabis cultivation modelled across the United States.
From: The greenhouse gas emissions of indoor cannabis production in the United States
The caption:
Fig. 2: Breakdown of life cycle GHG emissions contributions from indoor cannabis cultivation.
The caption:
Some technical details of growing conditions:
"ACH" apparently plays a big role:
Fig. 3: Sensitivity analysis of ACH.
From: The greenhouse gas emissions of indoor cannabis production in the United States
The caption:
Some commentary in the conclusion:
Was it Bob Dylan who said: "I would not feel so alone; everybody must get stoned?"
Whatever.
All this expense, for no other reason than the need to avoid reality...
History will not forgive us, nor should it.
Have a nice day tomorrow.
Ted Cruz Accuses Democrats of Trying to Cancel Poverty
Ted Cruz Accuses Democrats of Trying to Cancel Poverty(Borowitz Report.)
Poverty has been a part of American life since this country was founded, in 1776, but that apparently means nothing to the Democrat Cancel Culture Gang, Cruz said. In their intolerant view, poverty is somehow no longer appropriate.
He warned that, far from being Democrats ultimate goal, cancelling poverty is just the beginning.
If you read the fine print, they also want to cancel hunger and homelessness, he said. And, if youre used to living without health insurance, sorrythats yet another American tradition they want to cancel.
We should have seen this coming, he added. First they came for Mr. Potato Head.
Advice my parents gave me and advice I'll give my kids.
Admittedly, I am the "advice my parents gave," and not wishing to make fun of this rising great generation and regretting what mine has left for them, I nevertheless found this sadly amusing.
From the New Yorker: Advice My Parents Gave Me Versus Advice I Will Give My Kids
Advice I Will Give My Kids: Go to college only if youll major in science, engineering, or money. Its a bleak job market, and majoring in English literature or anything with the word English in it has been useless since the Taft Administration.
My Parents: Never show up to a party empty-handed.
Me: Never show up to a party. Send a text to the host twenty minutes before the party starts to say that youre sooooooo sorry to cancel but your stomach is feeling weird.
My Parents: To find a job, walk into the offices of ABC Newss This Week with George Stephanopoulos and ask for one.
Me: Apply to jobs via LinkedIn, ZipRecruiter, or nepotism. Write a cover letter and attach your résumé, then manually enter the same information through the companys portal, which looks as though it was designed in Microsoft Paint. Do this twenty times a day for two years, and youre bound to make it to a third round of phone interviews before getting ghosted.
My Parents: Dont put photos of yourself on the Internet. Youll get kidnapped!
Me: Post thousands of carefully curated photos of your life on Instagram so you can build a following and attract sponsors who reflect your core values, such as Bacardi and MeUndies.
My Parents: Spend your twenties finding true love within a two-mile radius of your village.
Me: Spend your twenties moving between L.A. and New York to figure out what you want in your ideal partner by dating all the worst people from both coasts and Austin, Texas.
My Parents: Show how much you appreciate your friends by making them elaborate, cellophane-wrapped gift baskets. Fill the baskets with gourmet biscuits, teas, and an ornate sugar spoon that says Gimme a little sugar, baby.
Me: Just Venmo them five dollars.
My Parents: Never date someone who rides a motorcycle.
Me: Never date someone who rides a unicycle ironically (unless the person got a MacArthur genius grant for it)...
Power Co-op Files Bankruptcy After $2.1 Billion ERCOT Bill
Power Co-op Files Bankruptcy After $2.1 Billion ERCOT BillSome excerpts:
Brazos Electric Power Cooperative filed its bankruptcy petition March 1 in the U.S. Bankruptcy Court for the Southern District of Texas. The company said it received a $2.1 billion charge from the Electric Reliability Council of Texas (ERCOT), the group that maintains and operates much of the states electricity grid. Brazos Electric is the wholesale energy provider for its 16-member cooperative.
Texas deregulated power market, which is not connected to other U.S. electricity transmission systems, means that most of the states power customers are not beholden to any one energy provider. Instead, customers can choose among dozens of electricity retailers on an open market.
Electricity generatorscompanies such as NRG and Vistraproduce power, which can then be sold by retail electric providers. Those retail companies include Griddy, which is being sued by the state attorney generals office for sending customers bills for as much as $5,000 for the cost of power during the weeklong storm.
State officials said they received more than 400 complaints about Griddy in less than two weeks. Texas Attorney General Ken Paxton in the lawsuit said Griddy deceived customers when it promised low wholesale energy prices...
Gee, isn't it amazing that Texas AG Ken Paxton is taking time out of trying to overthrow the government by committing election fraud in other people's states to pay attention to events in his own state?
Tales of Reliability, Climate, Water, and Energy on the Spanish Peninsula.
I'm not going to write very much about this paper, Sustainable Energy Transition Considering the WaterEnergy Nexus: A Multiobjective Optimization Framework, (Javier Tovar-Facio, Lidia S. Guerras, José M. Ponce-Ortega, and Mariano Martín ACS Sustainable Chemistry & Engineering 2021 9 (10), 3768-3780) in this post; other things are constraining my time.
It is about energy on the Iberian peninsula, specifically in Spain.
Nevertheless, in a sensible world, a graphic from the paper and a table from it would mean something fairly obvious, but unsurprisingly we don't live in a sensible world.
The graphic, figure 2 from the paper:
The key:
CA set of existing carbon power stations
CC set of existing combined cycle
CG set of existing cogeneration power plants
CS set of existing concentrated solar power
EO set of existing onshore wind power plants
HY set of existing hydroelectric power station
PV set of existing photovoltaic power plants
NU set of existing nuclear power plants
A table, table 1 from the paper:
Electricity is translating into a basic human right, in my view, since an indicator of poverty is the absence of electricity. However, for a sustainable world, we must produce electricity with the lowest reliable carbon impact.
There is one, and only one, system on the Spanish peninsula which is both reliable and low carbon.
It should be obvious, but it's not. And the fact that it's not as obvious as it should be is a reason we are now skirting 418 ppm of the dangerous fossil fuel waste carbon dioxide in the atmosphere, about 25 ppm higher than it was just 10 years ago.
Have a nice evening.
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